Microbiological, electronic microscopy evaluation of the action of Decasan® and Horosten® on bacteria

  • V.V. Bobyr The O.O. Bohomolets National Medical University in Kyiv
  • O.A. Nazarchuk The O.O. Bohomolets National Medical University in Kyiv
  • D.V. Paliy The M.I. Pyrohov National Medical University in Vinnytsya
  • O.V. Yatsula The M.I. Pyrohov National Medical University in Vinnytsya
Keywords: Decasan®, Horosten®, electronic microscopy, bacteria, Staphylococci, Salmonella

Abstract

Aim. The research was to study the action of Decasan® and Horosten® on ultra structure of Staphylococci and Salmonellas.

Material and Methods. The research was carried out on reference strains of S. aureus ATCC 25923, S. typhimurium 03270 and clinical isolates of S. aureus (n 53), S. typhymurium (n 15). The sensitivity of Staphylococci and Salmonellas to Decasan® (DC) and Horosten® (HR) was studied by means of standard serial dilution method. The influence of DC and HR on the ultra structure of bacteria was studied by means of electronic microscopy, using electronic microscope Jeol JEM 100 СХ II (Japan). Statistical analysis was carried out using Microsoft Excel 2007.

Results and Discussion. Minimal inhibitory concentrations of DC and HR against strains of S. aureus were found to be lower than 0,12 та 0,19 mkg/ml, respectively. Bactericidal action against S. aureus was found when 2,68±0,29 mkg/ml of antiseptics were used. S. typhimurium 03270 was found to be more resistant to DC and HR as their bactericidal concentrations were lower than 35,94±7,29 mkg/ml. No difference was found between sensitivity of S. aureus and S. typhimurium to DC and HR (р>0,05). Control samples of S. aureus obtained their typical ultra structure. The electronic research after two hour exposure to minimal inhibitory concentrations of DC and HR demonstrated huge morphological damage in bacteria. Changes of ultra structure in S. aureus and S. typhimurium were accompanied by cell polymorphism; loss of their typical structure; damage of their cell wall and its detachment with further protoplasmic efflux; depression of cell division; appearance of cells' lysis.

Conclusion. Antiseptic remedies DC and HR demonstrate high antimicrobial qualities against strains of S. aureus, S. typhimurium, with no difference in their effective inhibitory and bactericidal qualities (р>0,05); their inhibitory concentrations cause pronounced changes in ultra structure of bacteria.

References

Palіy G.K., Koget T.O., Paliy V.G. et. al. Antiseptics in prophylaxis and treatment of infection. K.: Zdorov'ya, 1997. 201 p.

Palіy G.K., Nazarchuk O.A., Gonchar O.O. et. al. The research of physical and chemical, antimicrobial qualities of "decamethoxin®" remedy. Medychna I klinichna khimiya 2016; 18, (1): 36-44.

Jung W, Koo H, Kim K, Shin S, Kim S, Park Y : Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus aureus and Escherichia coli. American Society for Microbiology 2008, 74 (7), 2171-2178. https://doi.org/10.1128/AEM.02001-07

Castillo J, Clape's P, Infante M. et al. : Comparative study of the antimicrobial activity of bis(Na-caproyl-L-arginine)-1,3-propanediamine dihydrochloride and chlorhexidine dihydrochloride against Staphylococcus aureus and Escherichia coli. Journal of Antimicrobial Chemotherapy, 2006, 57, 691-698. https://doi.org/10.1093/jac/dkl012

Nekrasova L.S., Svyta V.M., Glushkevych T.G. et al. Determination of microorganisms' sensitivity to antibacterial medicines: methodological guidelines MV 9.9.5 - 143. K.; 2007. 74 p.

Dykstra MJ, Reuss LE Biological electron microscopy: theory, techniques, and troubleshooting, Springer US, 2003, 2nd ed., 535 p. https://doi.org/10.1007/978-1-4419-9244-4

Sarkisova D.S., Petrova Yu.L. Microscopy Technique: Guideline for doctors and laboratory assistants. M.: Medicina, 1996. 544.

Yunkerov V.I., Grigorjev S.G., Rezvantsev M.V. Mathematical and statistical data processing of medical researches. SPb.: VMeedA, 2011. 318 p.

Published
2017-04-21
How to Cite
Bobyr, V., Nazarchuk, O., Paliy, D., & Yatsula, O. (2017). Microbiological, electronic microscopy evaluation of the action of Decasan® and Horosten® on bacteria. Acta Medica Leopoliensia, 23(1-2), 24-30. https://doi.org/10.25040/aml2017.01.024